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GI transit was analyzed via in vivo serial X-ray imaging prior to and following 3, 7, and 14 days of treatment. Balb/c mice received tri-weekly intraperitoneal 5-FU (23 mg/kg/d) administration with and without BGP-15 (15 mg/kg/d) for up to 14 days. In this study, we investigated whether the cytoprotectant, BGP-15, has a neuroprotective effect during 5-FU treatment. We have previously reported that long-term 5-fluorouracil (5-FU) administration results in enteric neuronal loss, acute inflammation and intestinal dysfunction. Chemotherapy-induced nausea, bloating, vomiting, constipation, and/or diarrhea can persist up to 10 years post-treatment. GI symptoms often lead to dose reduction, delays and cessation of treatment. Gastrointestinal (GI) side-effects of chemotherapy present a constant impediment to efficient and tolerable treatment of cancer.

7Head of Enteric Neuropathy Lab, Western Centre for Health Research and Education, Sunshine Hospital, Melbourne, VIC, Australia.

6Department of Medicine Western Health, The University of Melbourne, Melbourne, VIC, Australia.

5Department of Physiology, The University of Melbourne, Melbourne, VIC, Australia.

4Área de Farmacología y Nutrición y Unidad Asociada al Instituto de Química Médica del Consejo Superior de Investigaciones Científicas, Universidad Rey Juan Carlos, Alcorcón, Spain.

3Australian Institute for Musculoskeletal Science, Melbourne, VIC, Australia.

2Institute for Health & Sport, Victoria University, Melbourne, VIC, Australia.

1College of Health & Biomedicine, Victoria University, Melbourne, VIC, Australia.

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